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KMID : 0380020060210060433
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.433 ~ p.438
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In vivo Biological Function of a Fibrinolytic Enzyme after Oral Adminstration
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Lee Young-Hoon
Lee Sung-Ho
Park Ki-Hoon
Choi Young-Ju
Lee Sang-Won
Kim Cheol-Ho
Cho Soo-Jeong
Gal Sang-Wan
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Abstract
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A fibrinolytic enzyme gene (BCF-1) was subcloned to the pEB vector which is high expression vector in the Bacillus host. The enzyme was purified by using FPLC after ammonium sulfate precipitation. The enzyme was oral-administrated to the rat and checked the bleeding time, blood clotting time and fibrinolytic effect of the serum. In the bleeding time retardation test, it was longer about 1.7 fold in the feeding rat than without feeding. The serum of rat feeded with the enzyme had the fibrinolytic activity from 1 hour to 3 hours after oral-administration. After 3 hours from feeding, the fibrinolytic activity was decreased gradually. Also blood clotting time after bleeding was longer than that of control rat. The enzyme could be detected at band of 30,000 Da in the blood by western blotting. The enzyme was not harmful to the all internal organs of the rats. Taken together, the enzyme originated from B. subtilis BB-1 can be a candidate to develop the drug for thrombosis, arteriosclerosis and myocardial infarction.
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KEYWORD
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Fibrinolytic enzyme, oral-Administration, BCF-1, bleeding time, blood clotting time
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